Development of a continuous flow centrifugation rotor for detection of protozoa such as Cryptosporidium oocysts for drinking water.

Recovery and detection of Cryptosporidium parvum oocysts from water samples using continuous flow centrifugation.

Continuous flow centrifugation (CFC) was used in conjunction with immunomagnetic separation (IMS) and immunofluorescence microscopy (IFA) and nested PCR to recover and detect oocysts of Cryptosporidium parvum and cysts of Giardia intestinalis from 10L volumes of source water samples. Using a spiking dose of 100 oocysts, nine of 10 runs were positive by IFA, with a mean recovery of 4.4+/-2.27 oo...

Comparison of 12 Techniques for Detection of Cryptosporidium oocysts

Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples.

The development of a reliable method of using PCR for detection of Cryptosporidium oocysts in environmental samples with oligonucleotide primers which amplify a portion of the sequence encoding the small (18S) subunit of rRNA producing a 435-bp product was demonstrated. The PCR assay was found to provide highly genus-specific detection of Cryptosporidium spp. after release of nucleic acids from...

assessment of the effect of honey as a topical therapy for intra oral wound healing in rat.

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comparison of 12 techniques for detection of cryptosporidium oocysts

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